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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, pan (alpha, beta, gamma)

Anti -Actin, pan (alpha, beta, gamma)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Actin and myosin are the two major cytoskeleton proteins implicated in cellular movement, secretion, phagocytosis, and kinesis. Actin is one of the most conserved cellular protein. At least 6 actin isoforms have been identified by protein sequence analyses. Four actin isoforms represent the differentiation markers of muscle tissues. There are three a-actins: a-skeletal, a-cardiac, and a-smooth muscle), one b-actin (b-non-muscle), and two g-actins (g-smooth muscle and g-non-muscle). Actin isoform are ~90% conserved, except in the N-terminal 18aa (50-60%homology). Beta-actin protein and mRNA levels are often used as a reference for comparing changes in cellular protein/mRNA levels by Western or Northern blots.
Catalog #A0760-51J
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:10,000-1:50,000 (50-200ng control antigen/well)
Western Blot: 1500-1:2,000 (Chemiluminescence technique)
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to 11-aa-MAPS peptide (SGPSIVHRKCF) of human actin.
SpecificityRecognizes human Actin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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