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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Actin, pan (BSA & Azide Free)

Anti -Actin, pan (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH
Catalog #A0760-51X
ApplicationsSuitable for use in Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionImmunoprecipitation(Denatured verified): 2ug/mg protein lysate. Use Protein G.
Western Blot 0.5ug/ml for 2hrs at room temperature.
Immunohistochemistry (formalin/paraffin) 1:400 for 30 min. Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cells, skeletal muscle.
Cellular LocalizationCytoplasmic
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3F12
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See A0760-51
PurityPurified by Protein G affinity chromatography.
ImmunogenChicken gizzard actin
SpecificityReacts with all six known vertebrate isoforms of actin (MW~42kD). It also reacts with two cytoplasmic actins (beta, gamma) which are highly homologous to one another, but which differ from the muscle actins by about 25 amino acid residues. This antibody is highly recommended for monitoring total protein load on Western blots.
Species crossreactivityHuman, bovine, mouse, canine, porcine, rabbit, rat, chicken, Dictyostelium discoideum, Physarum polycephalum.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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