ARPC5, Recombinant, Human, aa1-151, His-tag (Actin-related Protein 2/3 Complex Subunit 5
Protein, Arp2/3 Complex 16kD Subunit 2, ARC16-2)
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| p16-ARC, also known as ARPC5, is a 151 amino acid subunit of the Arp2/3 complex. Thought to play a role in maintaining the integrity of Arp2/3, ARPC5 is a substrate for MAPKAPK-2 which, through phosphorylation of ARPC5, may participate in Arp2/3 regulatory functions and remodeling of the Actin cytoskeleton. Two isoforms of ARPC5 exist due to alternative splicing events. Purified by using conventional chromatography techniques. | | | Catalog # | A0760-89C | | Source | Recombinant correpsonding to aa1-151 of human ARPC5, fused to His-tag at N-terminus, expressed in E.coli. | | AA Sequences | MGSSHHHHHH SSGLVPRGSH MSKNTVSSAR FRKVDVDEYD ENKFVDEEDG GDGQAGPDEG EVDSCLRQGN MTAALQAALK NPPINTKSQA VKDRAGSIVL KVLISFKAND IEKAVQSLDK NGVDLLMKYI YKGFESPSDN SSAMLLQWHE KALAAGGVGS IVRVLTARKT V | | Molecular Weight | ~18.4kD (171aa), confirmed by MALDI-TOF | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~18.4kD | | Source | E.coli | | Purity | ~90% (SDS-PAGE) | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 1mM DTT, 0.1M sodium chloride, 50% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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