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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actinin, alpha 1 (Non-muscle)

Anti -Actinin, alpha 1 (Non-muscle)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors.
Catalog #A0761
ApplicationsSuitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications have not been tested.
Recommended DilutionsWestern Blot: 0.5-2ug/ml. Detects alpha-Actinin in RIPA lysates from human A431 cells.
Immunocytochemistry/Immunohistochemistry: 15ug/ml. Reported positive immunostaining for alpha-Actinin in MRC-5 cells fixed with methanol and also in frozen sections.
Optimal dilutions to be determined by the researcher.
Positive Antigen Control (Included)A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt x Mo
I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt x Mo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.    
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.T.02
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman alpha-Actinin
SpecificityRecognizes human alpha-Actinin 1 isoform (non-muscle) at 100kD. Species Crossreactivity: mouse and rabbit
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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