| The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors. |
|
| Catalog # | A0761 |
| Applications | Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Not suitable for use in Immunoprecipitation. Other applications have not been tested. |
| Recommended Dilutions | Western Blot: 0.5-2ug/ml. Detects alpha-Actinin in RIPA lysates from human A431 cells. |
| Immunocytochemistry/Immunohistochemistry: 15ug/ml. Reported positive immunostaining for alpha-Actinin in MRC-5 cells fixed with methanol and also in frozen sections. |
| Optimal dilutions to be determined by the researcher. |
| Positive Antigen Control (Included) | A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer. |
| Recommended Secondary Antibodies | I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo |
| I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt x Mo |
| I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt x Mo |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 0.T.02 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human alpha-Actinin |
| Specificity | Recognizes human alpha-Actinin 1 isoform (non-muscle) at 100kD. Species Crossreactivity: mouse and rabbit |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
