Anti -Actinin, alpha 1 (Alpha Actinin 1, ACTN1, Alpha Actinin Cytoskeletal Isoform,
F-actin Cross-linking Protein, FLJ40884, FLJ54432, Non-muscle alpha Actinin 1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
E B IP IC FC |
|
| Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross- link actin filaments. The rod domain determines the distance | | | between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1).Alpha-actinins are a multigene family of four actin- binding proteins related to dystrophin. Alpha-actinin-1 and -4 (ACTN1, ACTN4) are non-muscle isoforms version of ACTN. These isoform are commonly expressed in filopodia, lamellipodia, stress fibers, sites of adhesion and intercellular contacts (2). | | | | | | Applications: | | | Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. | | | Catalog # | A0761-01B | | Recommended Dilution | Flow Cytometry: 1:40 | | Western Blot: 1:500-1:1000 | | Immunoprecipitation: 1:40 | | Immunocytochemistry: 1:100-1:250 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 9H288 | | Host | Rabbit | | Source | Human | | Concentration | As reported | | Form | Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA. | | Purity | Supernatant | | Immunogen | A synthetic peptide corresponding to residues on the C-terminus of human Alpha-Actinin 1. | | Specificity | Recognizes human Alpha-Actinin 1. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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