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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actinin, alpha 1 (Alpha Actinin 1, alpha-Actinin-1, ACTN1, alpha-Actinin Cytoskeletal Isoform, F-actin Cross-linking Protein, FLJ40884, FLJ54432, Non-muscle alpha-Actinin-1)

Anti -Actinin, alpha 1 (Alpha Actinin 1, alpha-Actinin-1, ACTN1, alpha-Actinin
Cytoskeletal Isoform, F-actin Cross-linking Protein, FLJ40884, FLJ54432, Non-muscle
alpha-Actinin-1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IF
a-actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, a-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration and immune cell targeting. (1). The interaction of a-actinin with ICAM5 (intercellular adhesion molecule-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of a-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of a-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).
Catalog #A0761-02E
ApplicationsSuitable for use in Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionImmunofluorescence (IC): 1:25
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PuritySerum
ImmunogenSynthetic peptide corresponding to carboxy-terminal residues of human alpha-actinin-1, conjugated to KLH (P12814).
SpecificityReognizes endogenous levels of total human alpha-actinin-1 protein. Based on sequence homology, the antibody may cross-react with alpha-actinin isoforms 2 and 4. Species crossreactivity: mouse, rat, hamster and monkey.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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