Anti -Actinin, alpha 1 (Alpha Actinin 1, alpha-Actinin-1, ACTN1, alpha-Actinin
Cytoskeletal Isoform, F-actin Cross-linking Protein, FLJ40884, FLJ54432, Non-muscle
alpha-Actinin-1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
B IC IF |
|
| a-actinin belongs to the spectrin family of cytoskeletal proteins. It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer. More recently, a-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration and immune cell targeting. (1). The interaction of a-actinin with ICAM5 (intercellular adhesion molecule-5) helps to promote neurite outgrowth (2). In osteoblasts, interaction of a-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3). Isoforms 1 and 4 of a-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia and lamellipodia. The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1). | | | Catalog # | A0761-02E | | Applications | Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Immunofluorescence: 1:125 | | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | Not determined. | | Form | Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol. | | Purity | Serum | | Immunogen | Synthetic peptide corresponding to carboxy-terminal residues of human alpha-actinin-1. | | Specificity | Reognizes endogenous levels of total alpha-actinin-1 protein. Based on sequence homology, the antibody may cross-react with alpha-actinin isoforms 2 and 4. Species crossreactivity: Mouse, rat, hamster and monkey. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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