| The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors. |
|
| Catalog # | A0761-04 |
| Applications | |
| Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested. |
| Recommended Dilutions | Immunohistochemistry: 1ug/ml on frozen sections. Also suitable on paraffin sections. |
| Immunoprecipitation: 25ug/ml |
| Western Blot: 0.1-0.25ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Positive Control Included | A0761-04A Platelet Protein, Human |
| Supplied as a liquid in SDS sample buffer (100mM sodium chloride, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10ug/ml Bromophenol Blue sodium salt). |
| Use 5ul (25ug) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.125mg/ml |
| Form | Supplied as a liquid in PBS, 1mg/ml BSA, 0.01% sodium azide, before the addition of glycerol to 40%. |
| Purity | Purified by Immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to aa 28-38 (MGDYMAQEDDW) of human and rat actinin-4 and to aa 29-39 of mouse actinin-4. |
| Specificity | Recognizes human actinin-4. Does not cross-react with actinin-1, -2 or -3. Species Crossreactivity: mouse, rat, porcine and Chinese hamster. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
