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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Actinin, alpha 4

Anti -Actinin, alpha 4


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Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions. Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors.
Catalog #A0761-04
Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry: 1ug/ml on frozen sections. Also suitable on paraffin sections.
Immunoprecipitation: 25ug/ml
Western Blot: 0.1-0.25ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control IncludedA0761-04A Platelet Protein, Human
Supplied as a liquid in SDS sample buffer (100mM sodium chloride, 73mM TRIS/HCl pH 6.7, 10mM DTT, 8mM EDTA, 5% glycerol, 2% SDS, 10ug/ml Bromophenol Blue sodium salt).
Use 5ul (25ug) per lane for Western blotting of tricine gels (13% acrylamide) or 15% Laemmli gels.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.01% sodium azide, before the addition of glycerol to 40%.
PurityPurified by Immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 28-38 (MGDYMAQEDDW) of human and rat actinin-4 and to aa 29-39 of mouse actinin-4.
SpecificityRecognizes human actinin-4. Does not cross-react with actinin-1, -2 or -3. Species Crossreactivity: mouse, rat, porcine and Chinese hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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