| This antibody is specific for alpha isoform of skeletal and cardiac muscle actin. It does not crossreact with alpha isoform of smooth muscle actin. This antibody shows wide cross reactivity to other animal species including sheep, rabbit, guinea pig, rat, frog and snake. |
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| Catalog # | A0761-08 |
| Applications | Suitable for use in Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | Immunohistochemistry: Frozen and formalin-fixed, paraffin-embedded tissue sections. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Rhabdmyosarcoma/skeletal muscle |
| Expected Staining Pattern: Cytoplasmic |
| Epitope Retrieval: Not Required |
| Enzyme Digestion: Not Required |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| |
| Clone Type | Monoclonal |
| Isotype | IgM |
| Clone No | 6A4 |
| Host | Mouse |
| Source | Rabbit |
| Concentration | As reported |
| Form | Supplied as a liquid in PBS, pH 7.4, and 1% BSA with 0.1% sodium azide |
| Purity | Purified from mouse ascites |
| Immunogen | Purified rabbit striated muscle actin |
| Specificity | Recognizes Sarcomeric Actin Species Crossreactivity: human, sheep, rabbit, guinea pig, rat, frog and snake. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
