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Specifications

Clone Host Grade Applications Monoclonal Rabbit Serum B IP IH

Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibers, while ACTN3 expression is restricted to a subset of type 2 fibers (2).
Catalog #	A0761-09C
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000-1:10,000
	Immunohistochemistry: 1:100-1:250
	Immunoprecipitation: 1:40
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG
Clone No	9E121
Host	Rabbit
Source	Human
Concentration	Not determined
Form	Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Purity	Serum
Immunogen	A synthetic peptide corresponding to residues near the C-terminus of human Alpha-Actinin 2.
Specificity	Recognizes human Actinin, alpha 2 Species Crossreactivity: mouse and rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.