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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actinin, alpha 2, (CT)

Anti -Actinin, alpha 2, (CT)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Serum B IP IH
Alpha-actinin is a ubiquitously expressed protein found in numerous actin structures. It consists of an N-terminal actin binding domain, a central rod domain, and a C-terminal domain and functions as a homodimer to cross-link actin filaments. The rod domain determines the distance between cross-linked actin filaments and also serves as an interaction site for several cytoskeletal and signaling proteins (1). Alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibers, while ACTN3 expression is restricted to a subset of type 2 fibers (2).
Catalog #A0761-09C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:10,000
Immunohistochemistry: 1:100-1:250
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No9E121
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
PuritySerum
ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Alpha-Actinin 2.
SpecificityRecognizes human Actinin, alpha 2 Species Crossreactivity: mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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