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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Actin, Muscle

Anti -Actin, Muscle

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Supernatant IH
Muscle specific actin is a 42kD protein specific for actin present in skeletal, cardiac, and smooth muscle. This antibody may be useful in the study of tumors arising from these muscle cells, such as rhabdomyosarcomas, leiomyomas and leiomyosarcomas.
Catalog #A0761-11
ApplicationsSuitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry: Paraffin. 1:10-1:40, Frozen. Does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Clone 3H521 is reported to be suitable for use in Western blotting (1).
Optimal working dilutions to be determined by researcher.
Recommended positive control tissueSkeletal tissue.
Recommended Secondary ReagentsF(ab')2 rabbit anti-mouse IgG HRP
Recommended Negative ControlsMouse IgG1 Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H521
HostMouse
SourceHuman
ConcentrationAs reported
FormTissue Culture Supernatant. Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
PurityTissue culture supernatant.
ImmunogenMuscle actin, purified from human myocardium.
SpecificityRecognizes muscle specific actin. Clone 3H521 is specific for alpha actin and does not recognize beta actin. Species Crossreactivity: Clone 3H521 is reported to crossreact with canine (3), cavia (4), feline (5), lapine (6), Japanese macaque monkey (7), shrew (7), mouse (7), rat (7, 8), monkey (1) and chicken (9).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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