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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites B IP IH IF

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene.
Catalog #	A0762
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Western Blot:~100kD (SDS reducing gels).
	Immunofluorescence, Immunohistochemistry: Methanol fixed cells
	Note: For extensive dilution, protein containing or other stabilizing medium should be used.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	2Q1094
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as a liquid.
Purity	Ascites
Immunogen	Human platelet protein.
Specificity	Recognizes human alpha-actinin. Recognizes an epitope in the N-terminal region of alpha-actinin, probably between aa1-24.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.