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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.
Catalog #	A0762-01
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Western Blot: 3.5ug/ml
	Immunohistochemistry (Paraffin, frozen): 3.5-7ug/ml in formalin fixed, paraffin embedded sections and formalin or acetone fixed tissues. Boiling paraffin sections is required.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10K98
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with 1ml sterile PBS.
Purity	Purified by affinity chromatography.
Immunogen	Rabbit skeletal alpha-actinin
Specificity	Recognizes alpha-Actinin. Species Crossreactivity: human, mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.