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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites IH

Alpha-Actinin is a cytoskeletal and adhesion protein that provides a critical link between actin filaments and integrin adhesion receptors. It exists as an antiparallel homodimer which orients the actin-binding regions at opposite ends, allowing each dimer to bind two actin filaments leading to the formation of bundles. The N-terminal regions of both polypeptides, arranged in antiparallel orientation, comprise the actin-binding regions, while the C-terminal, larger parts consist of four spectrin-like repeats that interact to form a rod-like structure. The protein plays multiple roles in different cell types, being typical calcium-sensitive in non-muscle cells and calcium insensitive in muscle cells. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. However skeletal, cardiac, and smooth muscle alpha-actinins usually are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. The antibody described here is highly specific for alpha-skeletal muscle actinin and alpha-cardiac actinin.
Catalog #	A0762-01A
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (paraffin): 1:25-1:50
	Immunohistochemistry: Frozen
	Optimal dilutions to be determined by the researcher.
Positive Control	Skeletal muscle
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	11E7
Host	Mouse
Source	Rabbit
Concentration	Not determined.
Form	Supplied as a liquid, 0.05% sodium azide.
Purity	Ascites
Immunogen	Purified rabbit skeletal alpha-actinin. Cellular Localization: Cytoplasmic.
Specificity	Recognizes alpha-skeletal muscle actinin and alpha-cardiac actinin. Species Crossreactivity: feline, chicken, bovine, canine, goat, goldfish, hamster.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.