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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Actinin, alpha (alpha-Actinin, alpha-Actinin Cytoskeletal Isoform, F-actin Cross-linking Protein, Non-muscle alpha-Actinin)

Anti -Actinin, alpha (alpha-Actinin, alpha-Actinin Cytoskeletal Isoform, F-actin
Cross-linking Protein, Non-muscle alpha-Actinin)


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Clone Host Grade Applications
Monoclonal Mouse Supernatant B IH
Alpha-actinin is a cytoskeletal protein located in the Z band/disc region of skeletal muscle where it cross-links with F-actin. The presence of alpha-actinin can be related to a condition known as congenital nemaline myopathy. This antibody does not recognize alpha- actinin in type IIa or IIb human muscle fibers, but will help detect this protein in type 1 muscle fibers.
Catalog #A0762-01B
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:50-1:100
Immunohistochemistry: 1:40-1:80 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.
Positive ControlWestern Blot: Skeletal muscle
Immunohstochemistry (Frozen Unfixed): Normal human striated muscle frozen in isopentane chilled in liquid nitrogen. Staining pattern-striations localized to the Z disc in longitudinal sections.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone NoRBC2/1B6
ConcentrationNot determined
FormSupplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml dH2O.
ImmunogenCell membrane “ghosts” of human red blood cells.
SpecificityRecognizes alpha-actinin in human type 1 slow twitch muscle fibers and on red blood cell membranes. Species Crossreactivity: Chicken, canine and porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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