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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -Activation Transcription Factor 2, phosphorylated (Thr69/71) (ATF2, CREB P1)

Anti -Activation Transcription Factor 2, phosphorylated (Thr69/71) (ATF2, CREB P1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (24). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (24). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (24). In addition, mutations at these sites also reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
Catalog #A0855-42C
Applications Suitable for use in ELISA, Western Blotting and Immunohistochemistry (Paraffin sections). Other applications not tested.
Recommended DilutionWestern Blotting: 1:1000
Immunohistochemistry (Paraffin): 1:50
Optimal dilutions to be determined by the researcher.
Molecular Weight 70kD
See Control Antigen, A0855-42C1.
Storage and Stability May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA 50% glycerol. No preservative added.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr69 and Thr71 of human ATF-2.
SpecificityDetects endogenous levels of ATF-2 only when dually phosphorylated at both Thr69 and Thr71. It does not recognize ATF-2 singly phosphorylated at either Thr69 or Thr71. Species Crossreactivity: Mouse and rat.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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