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You are here:Home » Molecular Biology » MB-Transcription Factors » Activation Transcription Factor 2, phosphorylated (Thr69/71), Control Antigen (ATF2, CREB P1)

Activation Transcription Factor 2, phosphorylated (Thr69/71), Control Antigen (ATF2, CREB
P1)

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Specifications

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (24). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (24). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (24). In addition, mutations at these sites also reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2).
Catalog #A0855-42C1
Produced by immunizing rabbits with a synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr69 and Thr71 of human ATF-2.
Corresponding antibody, A0855-42C, detects endogenous levels of ATF-2 only when dually phosphorylated at both Thr69 and Thr71. It does not recognize ATF-2 singly phosphorylated at either Thr69 or Thr71. Species Crossreactivity Mouse and rat.
ApplicationsSuitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityPurified
ConcentrationAs reported
FormSupplied as a lyophilized powder.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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