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Activation Transcription Factor 2, phosphorylated (Thr69/71), Control Antigen (ATF2, CREB P1)
Activation Transcription Factor 2, phosphorylated (Thr69/71), Control Antigen (ATF2, CREB
P1)
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| The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2–4). Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2–4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 (2–4). In addition, mutations at these sites also reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 (2). | | | Catalog # | A0855-42C1 | | Produced by immunizing rabbits with a synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Thr69 and Thr71 of human ATF-2. | | Corresponding antibody, A0855-42C, detects endogenous levels of ATF-2 only when dually phosphorylated at both Thr69 and Thr71. It does not recognize ATF-2 singly phosphorylated at either Thr69 or Thr71. Species Crossreactivity | Mouse and rat. | | Applications | Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Other applications not tested. | | Recommended Dilution | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Purity | Purified | | Concentration | As reported | | Form | Supplied as a lyophilized powder. | | | Important Note | This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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