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Anti -Activation Transcription Factor 2, phosphorylated (Ser90) (ATF2, CREB P1)
Anti -Activation Transcription Factor 2, phosphorylated (Ser90) (ATF2, CREB P1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| Catalog # | A0855-42E | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | | | Western Blot | 0.5-2.0ug/ml Detects recombinant ATF2, phosphorylated by JNK1 1/SAPK1c, active, using a cold kinase assay. ATF2 and ATF2 phosphorylated by JNK1 1/SAPK1c, active were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-ATF2 (Ser90) (0.5ug/ml). Proteins were visualized using a rabbit anti-sheep secondary antibody conjugated to HRP and a chemiluminescence detection system. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | KLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Ser90 of human ATF2. The immunizing sequence is identical in human, mouse and rat. | | Specificity | Recognizes ATF2 phosphorylated by JNK1a1/SAPK1c, active. Species Crossreactivity: Human. Predicted to crossreact with mouse and rat, based on sequence homology. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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