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You are here:Home » Antibodies » Antibodies-Transcription Factors » Anti -Activation Transcription Factor 2, phosphorylated (Ser90) (ATF2, CREB P1)

Anti -Activation Transcription Factor 2, phosphorylated (Ser90) (ATF2, CREB P1)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
Catalog #A0855-42E
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot 0.5-2.0ug/ml Detects recombinant ATF2, phosphorylated by JNK1 1/SAPK1c, active, using a cold kinase assay. ATF2 and ATF2 phosphorylated by JNK1 1/SAPK1c, active were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho-ATF2 (Ser90) (0.5ug/ml). Proteins were visualized using a rabbit anti-sheep secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
PurityPurified by Protein G affinity chromatography.
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Ser90 of human ATF2. The immunizing sequence is identical in human, mouse and rat.
SpecificityRecognizes ATF2 phosphorylated by JNK1a1/SAPK1c, active. Species Crossreactivity: Human. Predicted to crossreact with mouse and rat, based on sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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