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You are here:Home » Molecular Biology » MB-Transcription Factors » Activating Transcription Factor 2, Recombinant, Human (ATF2, CREB P1)

Activating Transcription Factor 2, Recombinant, Human (ATF2, CREB P1)

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Specifications

Recombinant Human ATF-2 produced in E.Coli is a non-glycosylated, polypeptide chain encoding 19-96 amino acids and having a molecular mass of 57kD. The recombinant ATF-2 is manufactured as a maltose binding protein (MBP) fusion protein with an amino terminal polyhistidine tag. Human ATF-2 is purified by proprietary chromatographic techniques.
Catalog #A0855-42J
Unit DefinitionRecombinant human ATF2 is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with immunoprecipitated JNK from stimulated cells. This phosphorylation can be monitored by Western blot analysis using phosphorylation site specific antibody directed to ATF2 [pT71], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application. Please note: Kinase activity may vary depending on the substrate and reaction conditions.
StorageStore at 4°C if entire vial will be used within 1-2 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.
Molecular Weight57kD
SourceE. coli
Purity90% (SDS-PAGE)
ConcentrationNot determined
FormSupplied as a lyophilized powder without additives.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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