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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -Activating Transcription Factor 2 (ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558)

Anti -Activating Transcription Factor 2 (ATF2, activating transcription factor 2, HB16,
CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catalog #A0855-42K
Alternate Name ATF2, activating transcription factor 2, HB16, CREB2, TREB7, CRE-BP1, cAMP responsive element binding protein 2, MGC111558
Domain Information basic region leucin zipper
Gene Ontology Terms RNA polymerase II transcription factor activity; transcription co-activator activity
Application(s) Suitable for use in ELISA, Western Blot. Other applications not tested.
Western blot: Approx 60kD and 28kD bands seen in HeLa cell lysates. Recommended for use at 0.5-2ug/ml. Overnight incubation is recommended with this antibody. Please note that the appox 28kD band we observe maybe explained by the isoform described in Bailey et al [J. Clin. Endocr. Metab. 87: 1717-1728, 2002].
Staining (2ug/ml) of HeLa lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See A0855-42K-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenC-IVMAPSSQSQPSGS, C Terminus
SpecificityRecognizes Human, Rat ATF2
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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