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Activating Transcription Factor 2, NT (ATF2, CREB P1) (Biotin) Recombinant
Activating Transcription Factor 2, NT (ATF2, CREB P1) (Biotin) Recombinant
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| Activating Transcription Factor (ATF) Proteins are a family of cellular proteins that contain basic region leucine zipper (bZIP) DNA binding domains. The tax protein of human T cell leukemia virus type-1 (HTLV-1) stimulates in vitro DNA binding of several ATF bZIP domains by enhancing dimerization in absence of DNA resulting in viral transcription and cell transformation. Adenovirus E1a is a protein that stimulates transcription of early viral genes. E1a is not a sequence-specific DNA binding protein and therefore must target the viral promoter through a specific promoter bound protein called Activating Transcription Factor-2. | | | Catalog # | A0855-43A | | GST fusion-protein containing amino acids 19–96 of human ATF2 expressed in E. coli. | | Mr | 37kD | | Applications | Kinase Assay: 5ug was phosphorylated by JNK1a1/SAPK1c, active, J2500. This substrate can also be phosphorylated by JNK2a2/SAPK1a, active, J2600. | | Storage and Stability | For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. | | Molecular Weight | ~37kD | | Source | Recombinant, human fusion-protein expressed in E. coli. | | Purity | 70% (SDS-PAGE). Purified by glutathione-agarose chromatography. | | Concentration | ~1mg/ml | | Form | Recombinant protein in PBS. ~1mole Biotin is conjugated to 1mole of ATF2. | | Specificity | Recognizes ATF-2: 100%. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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