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Activating Transcription Factor 2, aa19-96, Recombinant, Human (ATF2, CREB P1)
Activating Transcription Factor 2, aa19-96, Recombinant, Human (ATF2, CREB P1)
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| Catalog # | A0855-43E | | Source | N-terminal GST fusion-protein containing amino acids 19-96 of human ATF2 expressed in E. coli. | | Applications | Suitable for use in Kinase Assay. Other applications not tested. | | Recommended Dilutions | Kinase Assay: 3um of ATF2 was phosphorylated by JNK 1a1/SAPK1c, active; this substrate can also be phosphorylated by JNK 2a2/SAPK1a, active. Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 35kD | | Source | E. coli | | Purity | 80% by SDS-PAGE. Purified by glutathione-agarose chromatography | | Concentration | ~2mg/ml | | Form | Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF and 50% glycerol. | | | | Important Note | This product is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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