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You are here:Home » Molecular Biology » MB-Transcription Factors » Activating Transcription Factor 2, aa19-96, Recombinant, Human (ATF2, CREB P1)

Activating Transcription Factor 2, aa19-96, Recombinant, Human (ATF2, CREB P1)

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Specifications

Catalog #A0855-43E
Source N-terminal GST fusion-protein containing amino acids 19-96 of human ATF2 expressed in E. coli.
ApplicationsSuitable for use in Kinase Assay. Other applications not tested.
Recommended DilutionsKinase Assay: 3um of ATF2 was phosphorylated by JNK 1a1/SAPK1c, active; this substrate can also be phosphorylated by JNK 2a2/SAPK1a, active. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight35kD
SourceE. coli
Purity80% by SDS-PAGE. Purified by glutathione-agarose chromatography
Concentration~2mg/ml
FormSupplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF and 50% glycerol.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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