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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -Activating Transcription Factor 2 (CT) (ATF2, cAMP Response Element Binding Protein 2, CRE BP1, CREB 2, CREB2, CREBP1, HB 16, HB16, MGC111558, mXBP, TREB 7, TREB7)

Anti -Activating Transcription Factor 2 (CT) (ATF2, cAMP Response Element Binding Protein
2, CRE BP1, CREB 2, CREB2, CREBP1, HB 16, HB16, MGC111558, mXBP, TREB 7, TREB7)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
The ATF/CREB family of DNA binding proteins consists of transcription factors whose activity is regulated by specific signaling pathways, including the stress/cytokine/radiation inducible pathways that function through SAPK1/JNK activation. Two threonine residues at positions 69 and 71 in the N-terminal region of ATF-2 are essential in mediating adenovirus E1A-inducible transcriptional activation. These residues are efficiently phosphorylated in vivo and are excellent substrates for SAPK1/JNK in vitro.
Catalog #A0855-43U
ApplicationsSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:10000
Immunohistochemistry (paraffin): 1:250-500
Immunocytochemistry: 1:250-500
Immunoprecipitation: 1:60
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa cell lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG
Clone No9i144
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues in C-terminus of human ATF-2.
SpecificityRecognizes human ATF-2. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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