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You are here:Home » Molecular Biology » MB-Transcription Factors » Activating Transcription Factor 2 Protein Kinase Substrate Fusion Protein (ATF2)

Activating Transcription Factor 2 Protein Kinase Substrate Fusion Protein (ATF2)

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Specifications

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 is known to interact with a variety of viral oncoproteins and cellular tumor suppressors and, more recently, has been shown to be a target of the SAPK/JNK and p38 MAP kinase signaling pathways (24). Various forms of cellular stress including genotoxic agents, inflammatory cytokines and UV irradiation stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (24). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress induced transcription by ATF-2 (24). In addition, mutations at these sites also reduce the ability of E1a and Rb to stimulate gene expression via ATF-2 (2). Activating Transcription Factor 2 (ATF-2) Fusion Protein serves as a useful substrate for SAPK/ JNK and p38 MAP kinases. It is expressed as a recombinant protein fusion containing ATF-2 residues 1996. It contains the N-terminal activation domain of ATF-2, which is regulated by phosphorylation of Thr69 and Thr71.
Catalog #A0855-44A
ApplicationSuitable for use in Kinase Assay. Other applications not tested.
Recommended DilutionsKinase Assay: 2ug/20ul reaction
A0855-44A can be phosphorylated by an upstream kinase in an in vitro kinase assay with 1X Kinase Buffer and 200uM ATP. After a 30-minute assay at 30°C, phosphorylation can be detected by Western blot with A0855-42B.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight34kD
SourceE. coli
Concentration~1mg/ml
FormSupplied as a liquid in 20mM Tris-HCl, pH 7.5, 50mM sodium chloride, 2mM Na2EDTA, 1mM dithiothreitol (DTT), 50% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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