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You are here:Home » Molecular Biology » MB-Transcription Factors » Activating Transcription Factor 2 Protein Kinase Substrate Fusion Protein (ATF2)

Activating Transcription Factor 2 Protein Kinase Substrate Fusion Protein (ATF2)


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The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 is known to interact with a variety of viral oncoproteins and cellular tumor suppressors and, more recently, has been shown to be a target of the SAPK/JNK and p38 MAP kinase signaling pathways (24). Various forms of cellular stress including genotoxic agents, inflammatory cytokines and UV irradiation stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (24). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress induced transcription by ATF-2 (24). In addition, mutations at these sites also reduce the ability of E1a and Rb to stimulate gene expression via ATF-2 (2). Activating Transcription Factor 2 (ATF-2) Fusion Protein serves as a useful substrate for SAPK/ JNK and p38 MAP kinases. It is expressed as a recombinant protein fusion containing ATF-2 residues 1996. It contains the N-terminal activation domain of ATF-2, which is regulated by phosphorylation of Thr69 and Thr71.
Catalog #A0855-44A
ApplicationSuitable for use in Kinase Assay. Other applications not tested.
Recommended DilutionsKinase Assay: 2ug/20ul reaction
A0855-44A can be phosphorylated by an upstream kinase in an in vitro kinase assay with 1X Kinase Buffer and 200uM ATP. After a 30-minute assay at 30°C, phosphorylation can be detected by Western blot with A0855-42B.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight34kD
SourceE. coli
FormSupplied as a liquid in 20mM Tris-HCl, pH 7.5, 50mM sodium chloride, 2mM Na2EDTA, 1mM dithiothreitol (DTT), 50% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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