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Activating Transcription Factor 2 Protein Kinase Substrate Fusion Protein (ATF2)
Activating Transcription Factor 2 Protein Kinase Substrate Fusion Protein (ATF2)
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| The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins (1). ATF-2 is known to interact with a variety of viral oncoproteins and cellular tumor suppressors and, more recently, has been shown to be a target of the SAPK/JNK and p38 MAP kinase signaling pathways (2–4). Various forms of cellular stress including genotoxic agents, inflammatory cytokines and UV irradiation stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 (2–4). Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress induced transcription by ATF-2 (2–4). In addition, mutations at these sites also reduce the ability of E1a and Rb to stimulate gene expression via ATF-2 (2). Activating Transcription Factor 2 (ATF-2) Fusion Protein serves as a useful substrate for SAPK/ JNK and p38 MAP kinases. It is expressed as a recombinant protein fusion containing ATF-2 residues 19–96. It contains the N-terminal activation domain of ATF-2, which is regulated by phosphorylation of Thr69 and Thr71. | | | Catalog # | A0855-44A | | Application | Suitable for use in Kinase Assay. Other applications not tested. | | Recommended Dilutions | Kinase Assay: 2ug/20ul reaction | | A0855-44A can be phosphorylated by an upstream kinase in an in vitro kinase assay with 1X Kinase Buffer and 200uM ATP. After a 30-minute assay at 30°C, phosphorylation can be detected by Western blot with A0855-42B. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 34kD | | Source | E. coli | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 50mM sodium chloride, 2mM Na2EDTA, 1mM dithiothreitol (DTT), 50% glycerol. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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