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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -ATF2, phosphorylated (Thr71) (Cyclic AMP-dependent Transcription Factor ATF-2, cAMP-dependent Transcription Factor ATF-2, Activating Transcription Factor 2, Cyclic AMP-responsive Element-binding Protein 2, CREB-2, cAMP-responsive Element-binding Protein 2

Anti -ATF2, phosphorylated (Thr71) (Cyclic AMP-dependent Transcription Factor ATF-2,
cAMP-dependent Transcription Factor ATF-2, Activating Transcription Factor 2, Cyclic
AMP-responsive Element-binding Protein 2, CREB-2, cAMP-responsive Element-binding Protein
2

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP IH
The transcription factor ATF-2 (also called CRE-BP1) is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CRE-dependent transcription. Phosphorylation of ATF-2 at Thr69 and Thr71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr69 and Thr71 to prolong the half-life of ATF-2. ATF-2 also functions as a histone acetyltransferase (HAT) by specifically acetylating histones H2B and H4 in vitro.
Catalog #A0855-44U
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1-4ug/ml
Immunoprecipitation: 10ug/ml
Immunohistochemistry: 20ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeLgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol.
PurityPurified by affinity chromatography.
ImmunogenSynthetic phosphopeptide corresponding to Thr71 from human ATF2.
SpecificityRecognizes human ATF-2 when phosphorylated at Thr71. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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