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Anti -Activating Transcription Factor 6 (ATF6, ATF6-alpha, ATF6A, Cyclic AMP-dependent Transcription Factor ATF-6 alpha)
Anti -Activating Transcription Factor 6 (ATF6, ATF6-alpha, ATF6A, Cyclic AMP-dependent
Transcription Factor ATF-6 alpha)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
B IP IH IF |
|
| ATF6, a basic-leucine zipper protein, was isolated by yeast interaction assay as a binding partner to SRF (serum response factor) (1). SRF is a transcription factor, which binds to the serum response element (SRE) in the c-fos promoter and regulates expression of the c-fos gene as well as other immediate-early genes and some tissue-specific genes. ATF6 has also been shown to interact with the endoplasmic reticulum (ER) stress response element (ERSE) (2). ATF6 constitutively expressed as a membrane-bound 90kD protein is directly converted to a 50kD protein in ER-stressed cells (3). When unfolded proteins accumulate in the ER, ATF6 is cleaved to release its cytoplasmic domain, which enters the nucleus (4). | | | Catalog # | A0855-70 | | Applications | Suitable for use in Western Blot, Immunoprecipitation, ChIP, Immunofluoresence and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1-5ug/ml | | Immunofluorescence: Methanol fixed at -20°C. | | Immunohistochemistry: Paraffin and frozen tissues. | | Optimal dilutions to be determined by the researcher. | | Recommended Positive Controls | NIH3T3 cells (mouse) | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG1,k | | Clone No | 4H18 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Partial protein containing aa1-273 of human ATF6. | | Specificity | Recognizes human Activating Transcription Factor 6 (ATF6). Both the full-length and cleaved forms are recognized at 90kD and 50-70kD, respectively. Species Crossreactivity: mouse, hamster, rabbit and rat | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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