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You are here:Home » Antibodies » Abs to Transcription Factors » Anti -Activating Transcription Factor 6 (ATF6, ATF6-alpha, ATF6A, Cyclic AMP-dependent Transcription Factor ATF-6 alpha)

Anti -Activating Transcription Factor 6 (ATF6, ATF6-alpha, ATF6A, Cyclic AMP-dependent
Transcription Factor ATF-6 alpha)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IC
ATF6, a basic-leucine zipper protein, was isolated by yeast interaction assay as a binding partner to SRF (serum response factor). SRF is a transcription factor, which binds to the serum response element (SRE) in the c-fos promoter and regulates expression of the c-fos gene as well as other immediate-early genes and some tissue-specific genes. ATF6 has also been shown to interact with the endoplasmic reticulum (ER) stress response element (ERSE). ATF6 constitutively expressed as a membrane-bound 90kD protein is directly converted to a 50kD protein in ER-stressed cells. When unfolded proteins accumulate in the ER, ATF6 is released and translocates to the golgi where it is cleaved into its active form which enters the nucleus and activates translation of UPR genes including XBP1.
Catalog #A0855-70C
ApplicationsSuitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 1-5ug/ml. Detects bands of ~90kD (full length ATF6) and ~50-70 kD (cleaved form of ATF6).
Immunohistochemistry: Paraffin and frozen sections
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6D101
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPartial human ATF6 (activating transcription factor 6) containing aa1-273.
SpecificityRecognizes human ATF6. Species Crossreactivity: mouse, rat, rabbit and hamster.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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