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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Activins are dimers consisting of two beta subunits. Several types of beta subunits have been described. The same beta subunits also occur in Inhibins as part of a heterodimer with an alpha subunit. The beta subunits are translated as a precursor form that includes an N-terminal pro region which is proteolytically cleaved to yield the mature C-terminal beta subunit.
Catalog #	A0855-89
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	Direct ELISA: 0.5-1.0ug/ml. The detection limit for recombinant human Activin A precursor is ~15ng/well.
	Western Blot: 1- 2ug/ml. The detection limit for recombinant human Activin A precursor is ~5ng/lane under non-reducing and reducing conditions.
	Optimal dilutions to be determined by the researcher.
Hybridoma	Mouse myeloma with B cells obtained from a mouse immunized with a DNA construct encoding the human Activin A pro region fused to mature human Activin beta B chain.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9H16
Host	Mouse
Source	Human
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	DNA construct encoding human Activin A precursor.
Specificity	Recognizes human Activin A Precursor.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.