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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B N

Activin B is a TGF-b family member that exhibits a wide range of biological activities including regulation of embryogenesis, osteogenesis, hematopoiesis, reproductive physiology and hormone secretion from the hypothalamic, pituitary and gonadal glands. Activin B, like certain other members of the TGF-b family, signals through the ActRII receptor (Activin Receptor type II). Human Activin B is a 26.0kD disulfide-linked homodimer consisting of two bB chains, each containing 115 amino acid residues.
Catalog #	A0855-89B
Applications	Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 0.5-1.0ug/ml. The detection limit is ~0.1ng/well.
	Western Blot: 1-2ug/ml. The detection limit is ~0.5ng/lane and 1ug/lane under non-reducing and reducing conditions.
	Neutralization: ~5-20ug/ml to neutralize 7.5ng/ml of human Activin AB on K562 cells.
	Optimal dilutions to be determined by the researcher.
Hybridoma	Mouse myeloma with B cells obtained from a mouse
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9H17
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, 5% trehalose.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant corresponding to human Activin B expressed in CHO cell.
Specificity	Recognizes human Activin B.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.