| Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated. |
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| Catalog # | A0856-05C |
| Applications | Suitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | Neutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml recombinant human Activin A to immobilized recombinant human Act RIIA/Fc Chimera (100ul of a 0.5ug/ml solution was coated in each well) in a functional ELISA assay. |
| Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.3ng/well. |
| Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane and 25ng/lane under non-reducing and reducing conditions, respectively. |
| Immunohistochemistry (Paraffin): 15ug/ml for 5-15um thick sections. For detection of labeling, the ABC technique using chromogenic substrates is recommended. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Source | Human |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS. |
| Purity | Purified by immunoaffinity chromatography. |
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| Immunogen | Sf 21-derived, recombinant human Activin RIIA extracellular domain. |
| Specificity | Recognizes human Activin Receptor Type IIA (RIIA). Shows < 2% crossreactivity with recombinant human Activin RIIB. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
