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You are here:Home » Antibodies » Antibodies-Activin Receptors » Anti -Activin Receptor Type IIA (RIIA)

Anti -Activin Receptor Type IIA (RIIA)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH N
Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated.
Catalog #A0856-05C
ApplicationsSuitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionNeutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml recombinant human Activin A to immobilized recombinant human Act RIIA/Fc Chimera (100ul of a 0.5ug/ml solution was coated in each well) in a functional ELISA assay.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.3ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~5ng/lane and 25ng/lane under non-reducing and reducing conditions, respectively.
Immunohistochemistry (Paraffin): 15ug/ml for 5-15um thick sections. For detection of labeling, the ABC technique using chromogenic substrates is recommended.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenSf 21-derived, recombinant human Activin RIIA extracellular domain.
SpecificityRecognizes human Activin Receptor Type IIA (RIIA). Shows < 2% crossreactivity with recombinant human Activin RIIB.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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