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You are here:Home » Antibodies » Abs to Activin Receptors » Anti -Activin Receptor Type IIB (RIIB)

Anti -Activin Receptor Type IIB (RIIB)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH N
Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated.
Catalog #A0856-10A
ApplicationsSuitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionNeutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml of recombinant human Activin A to immobilized recombinant human Act RIIB/Fc Chimera (100ul of a 2ug/ml solution was coated in each well) in an ELISA type assay.
Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.1ng/well.
Western Blot: 0.1-0.2ug/ml. The detection limit is ~1ng/lane under non-reducing and reducing conditions.
Immunohistochemistry (Paraffin): 5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose.
Reconstitute with 1ml sterile PBS.
PurityPurified by immunoaffinity chromatography.
Endotoxin<0.1EU/ug (LAL)
ImmunogenNS0-derived, recombinant human Activin Receptor Type IIB extracellular domain.
SpecificityRecognizes human Activin Receptor Type IIB (RIIB). Shows <1% crossreactivity with recombinant human Activin RIIA, recombinant human Activin RIA and recombinant human Activin RIB.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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