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You are here:Home » Antibodies » Abs to Centrosomal Proteins » Anti -ACTR1A (ARP1, Actin-Related Protein 1 Homolog A, Centractin alpha (yeast), Actin-RPV, Alpha-Centractin, ARP1, Centractin, Centrosome-associated Actin Homolog, CTRN1)

Anti -ACTR1A (ARP1, Actin-Related Protein 1 Homolog A, Centractin alpha (yeast),
Actin-RPV, Alpha-Centractin, ARP1, Centractin, Centrosome-associated Actin Homolog, CTRN1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50% amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis.
Catalog #A0857-40
Cellular Localization Cytoplasmic
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:8000.
Western Blot: 1-3ug/ml. Predicted molecular weight 43kD. Detects a band of ~ 45kD.
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide: YEEDGARSIHRKT representing the C-terminus of the human protein (residues 363-375) according to NP_005727.
SpecificityThis antibody may crossreact with ACTR1B Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (100% identity with immunogen) and Rat due to sequence homology.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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