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You are here:Home » Antibodies » Abs to Protein Kinases » Anti -AGK (Acylglycerol Kinase, Mitochondrial, hAGK, Multiple Substrate Lipid Kinase, HsMuLK, MuLK, Multi-substrate Lipid Kinase, MULK)

Anti -AGK (Acylglycerol Kinase, Mitochondrial, hAGK, Multiple Substrate Lipid Kinase,
HsMuLK, MuLK, Multi-substrate Lipid Kinase, MULK)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.
Catalog #A0858-54B
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:16,000
Western Blot: 1-3ug/ml, observed in human brain (substantia nigra, hippocampus) lysates on ~50kD bands
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to CDPRKREQMLTSP, from human AGK, at C-terminal (NP_060708.1).
SpecificityRecognizes human AGK. Species sequence homology: Mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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