| A disintegrin and metalloproteinase domain-containing protein 9 (ADAM 9) belongs to a family of transmembrane, membrane-anchored, disintegrin-containing proteins that are involved in several biological functions such as protein ectodomain shedding, cell fusion, and cell adhesion. ADAMs contain several characteristic domains, two of which, the protease and disintegrin domains, are noted for their homology to snake venom metalloprotease. The exact function of ADAM 9 is yet unknown, however it is widely distributed, is found within keratinocytes, and is ideally placed on the cell membrane, which some studies have suggested supports a role in cell migration and motility. Alternative splicing results in two isoforms; isoform 1 is found within the cell membrane and is considered a single-pass type 1 membrane protein, while isoform 2 is secreted. Mutations in ADAM 9 expression are causal to cone-rod dystrophy type 9 or CORD9 which is characterized by visible retinal pigment deposits and loss of cone photoreceptors leading and rod degeneration, ultimately resulting in severe loss of vision. |
|
| Catalog # | A0859-12 |
| Epitope | disintegrin domain |
| Molecular Weight | ~ 90kD observed. 2 isoforms are produced by alternative splicing: Isoform 1 at 91kD and Isoform 2 at 72kD. |
| Function | Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP. |
| Cofactor | Binds 1 zinc ion per subunit (Probable). |
| Size | 819 amino acids; 90556 Da |
| Subcellular Location | Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. |
| Tissue Specificity Widely expressed. Expressed in chondrocytes. Highest expression of isoform 2 found in liver and heart. |
| Applications | Suitable for use in Immunoblotting. Other applications have not been tested. |
| Recommended Dilution | Western Blot: 0.5ug/ml. Evaluated by Western Blot in human chondrocyte cell lysate. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | Human chondrocyte cell lysate |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.05% sodium azide. |
| Purity | Purified |
| Immunogen | KLH-conjugated linear peptide corresponding to the disintegrin domain of human ADAM 9. |
| Specificity | Recognizes human ADAM 9, Disintegrin Domain (A Disintigrin And Metalloproteinase 8, MDC9, Meltrin-g). Predicted to react with primate and chimpanzee based on 100% sequence homology. Species Crossreactivity: Mouse and rat (90% sequence homology). |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
