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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAM 10 Ectodomain (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 10)

Anti -ADAM 10 Ectodomain (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 10)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
ADAM10 is a member of the ADAM (a disintegrin and metalloprotease like domain) family. It cleaves the membrane-bound precursor of TNF-alpha at 76-Ala-|-Val-77 to its mature soluble form and is responsible for the proteolytic release of several cell-surface proteins, including heparin binding epidermal growth-like factor, ephrin-A2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). It contributes to the normal cleavage of the cellular prion protein and is involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicules, suggesting a vesicle-based protease activity.
Catalog #A0859-13C
ApplicationsSuitable for use in Direct ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsDirect ELISA: Shows ~20% crossreactivity with recombinant mouse ADAM10, ~5% crossreactivity with recombinant human TACE and <1% crossreactivity with recombinant human ADAM8, ADAM9, ADAM15 and BACE.
Western Blot: 1ug/ml under non-reducing conditions.
Immunohistochemistry: 5-15ug/ml with immersion fixed paraffin-embedded sections of human prostate cancer tissue.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa214-672 human ADAM10 ectodomain, expressed in Sf21 cells (O14672).
SpecificityRecognizes human ADAM10 ectodomain.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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