| ADAM12 (A disintegrin and metalloprotease domain 12; also meltrin- ) is a |
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| 120kD type I transmembrane glycoprotein member of the metzincin family of |
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| proteases. It is synthesized as a 909 amino acid (aa) preproprecursor that |
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| contains a 25kD proregion (aa 29-207) and a 92kD mature molecule. |
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| Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface |
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| expression. A 68kD soluble splice form exists in pregnancy where 36 aa |
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| replace the C-terminal 205 aa (705-909). This circulating ADAM12 isoform |
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| remains associated with its prodomain via noncovalent linkage, possibly acting |
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| as an enzyme regulator. Over aa 29-513, human ADAM12 shares 78% and |
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| 79% aa identity with bovine and mouse ADAM12, respectively. |
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| Catalog # | A0859-25K |
| Applications | Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | ELISA: 0.5-1.0ug/ml |
| Western Blot: 0.1- 0.2ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Sheep |
| Source | Human |
| Concentration | ~0.2mg/ml |
| Form | Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 40-50% glycerol, PBS. |
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| Purity | Purified by immunoaffinity chromatography. |
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| Immunogen | CHO cell-derived, recombinant human ADAM12 aa29-513; O43184 with a Glu36Gln substitution. |
| Specificity | Recognizes human ADAM12. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
