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A0859-25Q1 Mouse Anti-ADAM12 (MCMP, MLTN, MLTNA, MCMPMltna, A disintegrin and metalloproteinase domain 12, Meltrin alpha)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
O43184
Isotype
IgG1
Clone Number
11n1
Grade
Affinity Purified
Applications
IP
Crossreactivity
Hu
Gene ID
8038
Shipping Temp
Blue Ice
Storage Temp
-20°C

ADAM12 is a 120kD type I transmembrane glycoprotein member of the metzincin family of proteases. It is synthesized as a 909aa preproprecursor that contains a 25kD proregion aa29-207 and a 92kD mature molecule. Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression. A 68kD soluble splice form exists in pregnancy where 36aa replace the C-terminal 205 aa705-909. This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator. Over aa29-513, human ADAM12 shares 78-79aa identity with bovine and mouse ADAM12, respectively.

Applications
Suitable for use in Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Immunoprecipitation: 25ug/ml detects ADAM12 in conditioned cell culture medium spiked with recombinant human ADAM12. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Arg29-Ser513 from human ADAM12 expressed in CHO cells. Swiss/UniProt Accession: O43184.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human ADAM12. Does not react with recombinant human ADAM8, 9, 10, 15, 17, 19, 22, 23, 32 or 33.
Conjugates
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