Anti -ADAM12 (ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, A disintegrin and metalloproteinase
domain 12, Meltrin alpha)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
IP |
|
| ADAM12 is a 120kD type I transmembrane glycoprotein member of the metzincin family of proteases. It is synthesized as a 909aa preproprecursor that contains a 25kD proregion aa29-207 and a 92kD mature molecule. Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression. A 68kD soluble splice form exists in pregnancy where 36aa replace the C-terminal 205 aa705-909. This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator. Over aa29-513, human ADAM12 shares 78-79aa identity with bovine and mouse ADAM12, respectively. | | | Catalog # | A0859-25Q1 | | Applications | Suitable for use in Immunoprecipitation. Other applications not tested. | | Recommended Dilution | Immunoprecipitation: 25ug/ml detects ADAM12 in conditioned cell culture medium spiked with recombinant human ADAM12. | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Monoclonal | | Isotype | IgG1 | | Clone No | 11n1 | | Host | Mouse | | Source | Human | | Concentration | ~0.5mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS. | | Purity | Purified by Protein A/G affinity chromatography. | | Immunogen | Recombinant corresponding to Arg29-Ser513 from human ADAM12 expressed in CHO cells (O43184). | | Specificity | Recognizes human ADAM12. Does not react with recombinant human ADAM8, 9, 10, 15, 17, 19, 22, 23, 32 or 33. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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