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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAM12 (ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, A disintegrin and metalloproteinase domain 12, Meltrin alpha)

Anti -ADAM12 (ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, A disintegrin and metalloproteinase
domain 12, Meltrin alpha)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IP
ADAM12 is a 120kD type I transmembrane glycoprotein member of the metzincin family of proteases. It is synthesized as a 909aa preproprecursor that contains a 25kD proregion aa29-207 and a 92kD mature molecule. Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression. A 68kD soluble splice form exists in pregnancy where 36aa replace the C-terminal 205 aa705-909. This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator. Over aa29-513, human ADAM12 shares 78-79aa identity with bovine and mouse ADAM12, respectively.
Catalog #A0859-25Q1
ApplicationsSuitable for use in Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoprecipitation: 25ug/ml detects ADAM12 in conditioned cell culture medium spiked with recombinant human ADAM12.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No11n1
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein A/G affinity chromatography.
ImmunogenRecombinant corresponding to Arg29-Ser513 from human ADAM12 expressed in CHO cells (O43184).
SpecificityRecognizes human ADAM12. Does not react with recombinant human ADAM8, 9, 10, 15, 17, 19, 22, 23, 32 or 33.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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