Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 0.5ug/ml Western Blot: 0.1ug/ml Immunohistochemistry: 15ug/ml Immunoprecipitation: 25ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Sf 21-derived, recombinant human ADAM15 ectodomain.
Form
Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS.|
Purity
Purified by immunoaffinity chromatography.||
Specificity
Recognizes human ADAM15 ectodomain.