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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAM15 (a Disintegrin and Metalloprotease 15, Metargidin, MDC15)

Anti -ADAM15 (a Disintegrin and Metalloprotease 15, Metargidin, MDC15)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IC FC
ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.
Catalog #A0859-31B
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry and Direct ELISA. Other applications not tested.
Recommended DilutionsFlow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction 200ul.
Immunocytochemistry: 10ug/ml. Cells fixed with PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100 and 1% BSA. After blocking, cells were incubated with diluted A0859-31B followed by labeled anti-mouse IgG.
Western Blot: 1-2ug/ml. Detects ~5ng/lane of rhADAM15 under non-reducing conditions.
Immunoprecipitation: 25ug/ml
Direct ELISA: 1ug/ml detects ~0.3ng rhADAM15/well.
Optimal dilutions to be determined by the researcher.
Positive ControlMCF-7 cells
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9H33
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa207-696 from human ADAM15 ectodomain fused to the IgG Fc domain expressed in COS-7 cells. (Accession #Q13444)
SpecificityRecognizes human ADAM15 ectodomain.
Cross-reactivityShows 100% with rmADAM15 in Direct ELISA. Does not cross-react with rmADAM15, 10 , 28 and rhADAM8, 9, and 17 (TACE) in Western Blot. Does not cross-react with rhADAM8, 9,17, 28 and rmADAM9 and 10 in Direct ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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