| ADAM19 (a disintegrin and metalloprotease 19; also MADDAM and Meltrin- ) is a |
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| 95-100kD member of the M12B peptidase family of enzymes. It is expressed on |
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| multiple cell types including monocytes, fibroblasts, osteoblasts and dendritic cells. The |
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| human ADAM19 proenzyme is a 931 amino acid (aa) type I transmembrane protein. It |
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| contains a 178 aa cleavable proregion (aa 26-203) and a 497 aa extracellular domain |
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| (ECD) (aa 204-702). The ECD contains a metalloprotease domain (aa 211-409), a |
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| disintegrin region (aa 417-503) and a Cys-rich domain (aa 504-650). The ECD |
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| circulates in plasma bound to 700kD 2-Macroglobulin. One splice variant shows an |
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| alternate start site at Met71, while two isoforms show variations afterLys902. Over |
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| aa 205-702, human ADAM19 is 87% aa identical to mouse ADAM19. |
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| Catalog # | A0859-37 |
| Applications | Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. |
| Recommended Dilution | ELISA: 0.5-1.0ug/ml |
| Western Blot: 0.1-0.2ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Sheep |
| Source | Human |
| Concentration | ~0.2mg/ml |
| Form | Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with 40-50% glycerol, PBS. |
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| Purity | Purified by immunoaffinity chromatography. |
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| Immunogen | CHO cell-derived, rhADAM19; aa 26-501; with a Ser32 deletion and a Lys33Arg substitution. |
| Specificity | Recognizes human ADAM19. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
