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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAM 21 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 21)

Anti -ADAM 21 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 21)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IC
Catalog #A0859-40
ApplicationsSuitable for use in Western Blot, Immunocytochemistry, Immunhistochemistry and ELISA. Not suitable for Flow Cytometry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:1000. 5% nonfat milk in PBS for blocking.
Variable sizes on gel expected, as there are several processed forms of the protein: 110kD for the precursor, 90kD at the MMP domain, and various uncharacterized smaller forms.
Immunohistochemistry: 1:100
Immunocytochemistry: 1:100 Tissues should be either fresh frozen or fixed in cold acetone. Tissues should be blocked in 10% Normal Goat serum in PBS. Antibody should be incubated in 10% Normal Goat Serum at a dilution of 1:200 and incubated 2 hours at RT or overnight at 4ºC. The antibody can be visualized by fluorescence.
Optimal dilutions to be determined by the researcher.
Positive ControlTestes extract or tissue
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~2mg/ml
FormSupplied as a liquid in PBS, 1% BSA.
PurityPurified by affinity chromatography.
ImmunogenSynthetic peptide corresponding to 13aa of human ADAM 21 at the C-terminal.
SpecificityRecognizes human ADAM 21. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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