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Anti -ADAM 21 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 21)
Anti -ADAM 21 (Kuzbanian, KUZ, MADM, a Disintegrin and Metalloprotease 21)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
B IH IC |
|
| Catalog # | A0859-40 | | Applications | Suitable for use in Western Blot, Immunocytochemistry, Immunhistochemistry and ELISA. Not suitable for Flow Cytometry. Other applications have not been tested. | | Recommended Dilutions | Western Blot: 1:1000. 5% nonfat milk in PBS for blocking. | | Variable sizes on gel expected, as there are several processed forms of the protein: 110kD for the precursor, 90kD at the MMP domain, and various uncharacterized smaller forms. | | Immunohistochemistry: 1:100 | | Immunocytochemistry: 1:100 Tissues should be either fresh frozen or fixed in cold acetone. Tissues should be blocked in 10% Normal Goat serum in PBS. Antibody should be incubated in 10% Normal Goat Serum at a dilution of 1:200 and incubated 2 hours at RT or overnight at 4ºC. The antibody can be visualized by fluorescence. | | Optimal dilutions to be determined by the researcher. | | Positive Control | Testes extract or tissue | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~2mg/ml | | Form | Supplied as a liquid in PBS, 1% BSA. | | Purity | Purified by affinity chromatography. | | Immunogen | Synthetic peptide corresponding to 13aa of human ADAM 21 at the C-terminal. | | Specificity | Recognizes human ADAM 21. Species Crossreactivity: mouse | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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