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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAM 23 (A Disintegrin And Metalloproteinase 23) (Biotin)

Anti -ADAM 23 (A Disintegrin And Metalloproteinase 23) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
ADAM23 is a 100kD member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum and serves as a counter-receptor for alphav, beta3 integrin. The human ADAM23 proprecursor is a 773aa type I transmembrane (TM) protein. It contains a 227aa
cleavable proregion (aa60-286) and a 506aa extracellular domain (ECD) (aa287-792) that is part of a 70kD mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa 299-494), an integrin-binding disintegrin region (aa511-585) and a Cys-rich domain (aa589-611). Two splice variants exist. One shows an in-frame 46aa substitution for aa787-832 that generates a soluble form, while a second shows an in-frame 31aa TM substitution for aa787-817. Over aa1-585, human ADAM23 is 92% aa identical to mouse ADAM23.
Catalog #A0859-41C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.2ug/ml. The detection limit is ~10ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS (20mM Trizma base, 150mM sodium chloride), pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.05mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS (20mM Trizma base, 150mM sodium chloride), pH 7.3, 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenPurified, recombinant corresponding to aa60-585 of human ADAM23 expressed in CHO cells (Accession # O75077).
SpecificityRecognizes human ADAM23. Shows <5% crossreactivity with recombinant human ADAM8, -9 and -22.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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