| ADAM23 is a 100kD member of the M12B peptidase family of enzymes. It is expressed on fetal neurons in the hippocampus and cerebellum and serves as a counter-receptor for alphav, beta3 integrin. The human ADAM23 proprecursor is a 773aa type I transmembrane (TM) protein. It contains a 227aa |
|
| cleavable proregion (aa60-286) and a 506aa extracellular domain (ECD) (aa287-792) that is part of a 70kD mature molecule. The ECD contains a nonfunctional metalloprotease domain (aa 299-494), an integrin-binding disintegrin region (aa511-585) and a Cys-rich domain (aa589-611). Two splice variants exist. One shows an in-frame 46aa substitution for aa787-832 that generates a soluble form, while a second shows an in-frame 31aa TM substitution for aa787-817. Over aa1-585, human ADAM23 is 92% aa identical to mouse ADAM23. |
|
| Catalog # | A0859-41C |
| Applications | Suitable for use in Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 0.1-0.2ug/ml. The detection limit is ~10ng/lane under non-reducing and reducing conditions. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS (20mM Trizma base, 150mM sodium chloride), pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Sheep |
| Source | Human |
| Concentration | ~0.05mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS (20mM Trizma base, 150mM sodium chloride), pH 7.3, 0.1% BSA. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Purified, recombinant corresponding to aa60-585 of human ADAM23 expressed in CHO cells (Accession # O75077). |
| Specificity | Recognizes human ADAM23. Shows <5% crossreactivity with recombinant human ADAM8, -9 and -22. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
