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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAM 32 (ADAM metallopeptidase domain 32, ADAM 32 precursor, A disintegrin and metalloprotease domain 32, A disintegrin and metalloproteinase domain 32, FLJ26299, FLJ29004, UNQ5982/PRO21340)

Anti -ADAM 32 (ADAM metallopeptidase domain 32, ADAM 32 precursor, A disintegrin and
metalloprotease domain 32, A disintegrin and metalloproteinase domain 32, FLJ26299,
FLJ29004, UNQ5982/PRO21340)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified E B IP
ADAM32 (a disintegrin and metalloprotease domain 32) is a 90-100kD member of the M12B peptidase family of proteins. It is expressed on sperm found in the testis, epididymis and vas deferens. The human ADAM32 proprecursor is a 771aa type I transmembrane protein. It contains a 158 aa proregion (aa17-174) and a 508aa extracellular domain (ECD) (aa175-682). The ECD contains a nonfunctional metalloprotease domain (aa186-383), an integrin-binding disintegrin region (aa391-479), a Cys-rich segment (aa480-502) and an EGF-like domain
(aa 622-654). In the testis, mature ADAM32 is approximately 98kD in size; in the epididymis, cleavage occurs after the metalloprotease domain to generate a 44kD product. There are two potential splice events that show a deletion of aa306-401 plus a 53aa substitution for the N-terminal 46aa. Over aa17-476, human ADAM32 is 66% aa identical to mouse ADAM32.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Catalog #A0859-41M
Recommended DilutionELISA: 0.5-1.0ug/ml
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenCHO cell-derived, recombinant human ADAM32 aa17-476; Q8TC27.
SpecificityRecognizes human ADAM32.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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