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Abs to Matrix Metalloproteinases
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Anti -ADAM 32 (ADAM metallopeptidase domain 32, ADAM 32 precursor, A disintegrin and metalloprotease domain 32, A disintegrin and metalloproteinase domain 32, FLJ26299, FLJ29004, UNQ5982/PRO21340)
Anti -ADAM 32 (ADAM metallopeptidase domain 32, ADAM 32 precursor, A disintegrin and
metalloprotease domain 32, A disintegrin and metalloproteinase domain 32, FLJ26299,
FLJ29004, UNQ5982/PRO21340)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
E B IP |
|
| ADAM32 (a disintegrin and metalloprotease domain 32) is a 90-100kD member of the M12B peptidase family of proteins. It is expressed on sperm found in the testis, epididymis and vas deferens. The human ADAM32 proprecursor is a 771aa type I transmembrane protein. It contains a 158 aa proregion (aa17-174) and a 508aa extracellular domain (ECD) (aa175-682). The ECD contains a nonfunctional metalloprotease domain (aa186-383), an integrin-binding disintegrin region (aa391-479), a Cys-rich segment (aa480-502) and an EGF-like domain | | | (aa 622-654). In the testis, mature ADAM32 is approximately 98kD in size; in the epididymis, cleavage occurs after the metalloprotease domain to generate a 44kD product. There are two potential splice events that show a deletion of aa306-401 plus a 53aa substitution for the N-terminal 46aa. Over aa17-476, human ADAM32 is 66% aa identical to mouse ADAM32. | | | | | | Applications: | | | Suitable for use in ELISA and Western Blot. Other applications not tested. | | | Catalog # | A0859-41M | | Recommended Dilution | ELISA: 0.5-1.0ug/ml | | Western Blot: 1.0ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | CHO cell-derived, recombinant human ADAM32 aa17-476; Q8TC27. | | Specificity | Recognizes human ADAM32. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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