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You are here:Home » Antibodies » Abs to Matrix Metalloproteinases » Anti -ADAM 33 (A Disintegrin and Metalloprotease 33, A Disintegrin and Metalloproteinase Domain 33, HGNC:15478, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC71889, Disintegrin and Reprolysin Metalloproteinase Family Protein, Metalloprotease Disintegrin)

Anti -ADAM 33 (A Disintegrin and Metalloprotease 33, A Disintegrin and Metalloproteinase
Domain 33, HGNC:15478, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC71889, Disintegrin
and Reprolysin Metalloproteinase Family Protein, Metalloprotease Disintegrin)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IP FC
ADAM33 is a widely expressed member of the ADAM family of transmembrane proteins. It contains a metalloprotease-like domain, disintegrin cell adhesion domain, and cysteine rich domain. ADAM33 is involved in the maintenance of airway function, and ADAM33 polymorphisms have been associated with asthma susceptibility and bronchial hyperresponsiveness. Within the mature ectodomain, human and mouse ADAM33 share 79% amino acid sequence identity.
Catalog #A0859-42B
ApplicationsSuitable for use in Flow Cytometry, Direct ELISA, and Immunoprecipitation. Other applications not tested.
Recommended DilutionsFlow Cytometry: 25ug/ml. 10ul labels 1-5x10e5 A549 cells in a total reaction volume 200ul. For intracellular staining, cells must first be fixed and permeabilized using 4% paraformaldehyde, 0.1% saponin in PBS.
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human ADAM33 is ~10ng/well.
Immunoprecipitation: 25ug/ml to immunoprecipitate recombinant human ADAM33 from conditioned cell culture media.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No9H38
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to aa30-501 from human ADAM33, expressed in CHO cells (Q9BZ11).
SpecificityRecognizes human ADAM33.
Species CrossreactivityRecombinant mouse ADAM33, <5%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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