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You are here:Home » Antibodies » Abs to Proteins » Anti -ADAP (SLAP-130, Fyb, FYN Binding Protein, Adhesion and Degranulation Promoting Adaptor Protein, B630013F22Rik, FYB-120/130, FYN-binding Protein, FYN-T-binding Protein, p120/p130, SLP-76-associated Phosphoprotein)

Anti -ADAP (SLAP-130, Fyb, FYN Binding Protein, Adhesion and Degranulation Promoting
Adaptor Protein, B630013F22Rik, FYB-120/130, FYN-binding Protein, FYN-T-binding Protein,
p120/p130, SLP-76-associated Phosphoprotein)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IP
FYB (FYN binding protein) was initially identified as a molecular adaptor that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of FYB, ultimately activates the NFkB family of transcription factors. More recent experiments have shown that FYB controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bclx(L), suggesting that FYB regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of FYB are known to exist.
Catalog #A0859-43
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsWestern Blot: 0.5-2.0ug/ml detected ADAP/SLAP-130/Fyb in RIPA lysates from RAW 264.7 macrophage cells. RAW 264.7 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A0859-43. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitated ADAP/SLAP-130/Fyb from 500ug of RAW 264.7 macrophage RIPA lysate.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40J: IgG, Fc (HRP) Pab Gt x Rb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide corresponding to aa755-768 DNDGEIYDDIADGC of mouse Fyn binding protein (ADAP/SLAP-130/Fyb), conjugated to KLH.
SpecificityRecognizes mouse ADAP/SLAP-130/Fyb, Mr ~130kD. An unknown protein was also detected, Mr ~40kD that is also inhibited by the immunizing peptide. Species Sequence Homology: human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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