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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

Human SH2 domain containing leukocyte protein-76-Associated Phosphoprotein of 130kD (SLAP-130), also known as Fyb-120 and ADAP, is an 86kD, 783 amino acid, cytoplasmic protein that runs anomalously as a 120-130kD protein in SDS-PAGE. Human SLAP-130 is a hematopoietic-specific adaptor which associates with other cytoplasmic proteins such as SH2-containing Leukocyte Phosphoprotein of 76kD (SLP-76). Adaptors integrate signals from surface receptors and are essential components of many immune cell-signaling cascades. There is at least one known 46 aa longer alternatively-spliced SLAP-130 isoform. Over the region used as immunogen, SLAP-130 is 66% and 76% identical to the corresponding canine and mouse proteins, respectively.
Applications:
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human SLAP-130. The detection limit for rhSLAP-130 is approximately 1ng/lane under non-reducing and reducing conditions.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human SLAP-130. The detection limit for rhSLAP-130 is approximately 0.2ng/well.
Optimal dilutions should be determined by each laboratory for each application.
Catalog #	A0859-43A
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS, with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Purity	Purified by human SLAP-130 affinity chromatography.
Immunogen	E. coli-derived rhSLAP-130
Specificity	Selected for its ability to recognize human SLAP-130 in direct ELISAs and Western Blots.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.