Anti -Adaptor Protein, Phosphotyrosine Interaction, PH Domain and Leucine Zipper
Containing 1 (APPL1, DIP13alpha, Signaling Adaptor Protein DIP13alpha, adaptor protein
containing pH Domain, PTB domain and leucine zipper motif)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B IP IH |
|
| APPL1, an adaptor protein containing an NH2-terminal Bin/Amphiphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain and a COOH-terminal phosphotyrosine binding (PTB) domain, was originally identified as an interacting partner of Akt in a yeast two-hybrid assay using Akt2 as a bait. APPL1 binds to a number of cell surface receptors (TrkA, DCC, adiponectin, FSH) and intracellular signaling molecules (small GTPase Rab5, GIPC and inositol 5- phosphatase, suggesting that APPL1 may act as a common relay to coordinate diverse signaling cascades. APPL1 potentiates insulin-mediated Akt activation by counteracting the effect of the Akt inhibitor TRB3. | | | Catalog # | A0859-44C | | Applications | Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested. | | Recommended Dilution | ELISA:0.5-1ug/ml | | Western Blot:0.1-0.2ug/ml | | Immunohistochemistry:1-2ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant full-length human APPL1 expressed in E.coli. | | Specificity | Recognizes human APPL1. Species Cross-Reactivity: monkey, mouse, rat. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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