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You are here:Home » Antibodies » Abs to Cell Surface Proteins » Anti -APPL1 (DCC-interacting Protein 13-alpha, Dip13-alpha, Adapter Protein Containing PH Domain, PTB Domain and Leucine Zipper Motif 1, APPL, DIP13A, KIAA1428)

Anti -APPL1 (DCC-interacting Protein 13-alpha, Dip13-alpha, Adapter Protein Containing PH
Domain, PTB Domain and Leucine Zipper Motif 1, APPL, DIP13A, KIAA1428)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
APPL1, an adaptor protein containing an NH2-terminal Bin/Amphiphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain and a COOH-terminal phosphotyrosine binding (PTB) domain, was originally identified as an interacting partner of Akt in a yeast two-hybrid assay using Akt2 as a bait. APPL1 binds to a number of cell surface receptors (TrkA, DCC, adiponectin, FSH) and intracellular signaling molecules (small GTPase Rab5, GIPC and inositol 5- phosphatase, suggesting that APPL1 may act as a common relay to coordinate diverse signaling cascades. APPL1 potentiates insulin-mediated Akt activation by counteracting the effect of the Akt inhibitor TRB3.
Catalog #A0859-44E
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:64,000
Western Blot: 0.3-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGoat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to C-NRYSRLSKKRENDK, from APPL1, at the internal region of the protein (NP_036228.1).
SpecificityRecognizes APPL1. Species sequence homology: Bovine, canine, human, mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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