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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Adducin, phosphorylated (Ser 724) (phosphoAdducin)

Anti -Adducin, phosphorylated (Ser 724) (phosphoAdducin)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
Adducin is a heteromeric protein with subunits containing a COOH-terminal myristoylated alkaline-rich C kinase substrate (MARKS-related) domain. The MARKS-related domain, present in a, b, and g adducin subunits, binds calmodulin and contains the major phosphorylation site for protein kinase C (PKC)(3). Adducin is localized at spectrin-actin junctions in erythrocyte membrane skeletons and co-localizes with spectrin at sites of cell-cell contact in epithelial cells(1) and in dentritic spines of hippocampal neurons(2). Studies show that PKC phosphorylation of the MARKS-related domain of adducin regulates actin capping and abolishes the activity of adducin in recruiting spectrin to ends and sides of actin filaments.(3)
Catalog #A0859-55
ApplicationsSuitable for use in Immunoprecipitation, Immunocytochemistry and Immunoblot. Other applications not tested.
Recommended DilutionImmunoblot Analysis: 0.5-2ug/ml detected phospho-Adducin (S724) in lysates from human red blood cells stimulated with PMA. Non-stimulated and PMA-stimulated human red blood cell lysate, was resolved by electrophoresis, transferred to nitrocellulose and probed with A0859-55 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation: Reported to immunoprecipitate Adducin, phosphorylated (Ser 724)
Immunocytochemistry: Reported to stain Adducin, phosphorylated (Ser 724)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4i24
FormSupplied as a liquid in 100ul of 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenKLH conjugated synthetic peptide (FRTP[pS]FLKK) corresponding to aa720-728 of rat phospho-a-Adducin (S724).
SpecificityRecognizes phospho-Adducin isoforms ranging in Mr from 80120kD. Species crossreactivity: Rat, mouse and human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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