| Adducin is a heteromeric protein with subunits containing a COOH-terminal myristoylated alkaline-rich C kinase substrate (MARKS-related) domain. The MARKS-related domain, present in a, b, and g adducin subunits, binds calmodulin and contains the major phosphorylation site for protein kinase C (PKC)(3). Adducin is localized at spectrin-actin junctions in erythrocyte membrane skeletons and co-localizes with spectrin at sites of cell-cell contact in epithelial cells(1) and in dentritic spines of hippocampal neurons(2). Studies show that PKC phosphorylation of the MARKS-related domain of adducin regulates actin capping and abolishes the activity of adducin in recruiting spectrin to ends and sides of actin filaments.(3) |
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| Catalog # | A0859-55 |
| Applications | Suitable for use in Immunoprecipitation, Immunocytochemistry and Immunoblot. Other applications not tested. |
| Recommended Dilution | Immunoblot Analysis: 0.5-2ug/ml detected phospho-Adducin (S724) in lysates from human red blood cells stimulated with PMA. Non-stimulated and PMA-stimulated human red blood cell lysate, was resolved by electrophoresis, transferred to nitrocellulose and probed with A0859-55 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. |
| Immunoprecipitation: Reported to immunoprecipitate Adducin, phosphorylated (Ser 724) |
| Immunocytochemistry: Reported to stain Adducin, phosphorylated (Ser 724) |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 4i24 |
| Host | Mouse |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 100ul of 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide before the addition of glycerol to 30%. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | KLH conjugated synthetic peptide (FRTP[pS]FLKK) corresponding to aa720-728 of rat phospho-a-Adducin (S724). |
| Specificity | Recognizes phospho-Adducin isoforms ranging in Mr from 80–120kD. Species crossreactivity: Rat, mouse and human. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
