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You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Adducin, alpha, phosphorylated, Ser481 (PhosphoAdducin)

Anti -Adducin, alpha, phosphorylated, Ser481 (PhosphoAdducin)

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IP IH IC
Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; and , or and subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5).
Applications:
Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Catalog #A0859-55B
Recommended DilutionWestern Blotting: 1:20,000
Immunohistochemistry: 1:250-500
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Clone TypeMonoclonal
IsotypeIgG
Clone No6k10
HostRabbit
SourceHuman
FormSupplied as a liquid
PuritySupernatant
ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser481 of human alpha Adducin
SpecificityRecognizes human alpha Adducin when phosphorylated at Ser481.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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