You are here:Home
»
Antibodies
»
Abs to Actin Related Proteins
»
Anti -Adducin, alpha, phosphorylated, Ser481 (PhosphoAdducin)
Anti -Adducin, alpha, phosphorylated, Ser481 (PhosphoAdducin)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH IC |
|
| Adducin is a membrane skeletal protein that binds to actin filaments (F-actin) and promotes the association of spectrin with F-actin to form a spectrin-actin meshwork beneath plasma membranes (1-2). Adducin is a heterodimeric protein that consists of related subunits; and , or and subunits (3). Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinase C (PKC), and protein kinase A (PKA) (4). PKA specifically phosphorylates Ser408, Ser436, and Ser481 located in the neck domain of alpha Adducin. Phosphorylation of Adducin alpha by Rho-kinase at Thr445 and Thr480 enhances the F-actin-binding activity of Adducin alpha (5). | | | | | | Applications: | | | Suitable for use in Western Blotting, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. | | | Catalog # | A0859-55B | | Recommended Dilution | Western Blotting: 1:20,000 | | Immunohistochemistry: 1:250-500 | | Immunocytochemistry: 1:100-250 | | Immunoprecipitation: 1:50 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 6k10 | | Host | Rabbit | | Source | Human | | Form | Supplied as a liquid | | Purity | Supernatant | | Immunogen | A synthetic phospho-peptide corresponding to residues surrounding Ser481 of human alpha Adducin | | Specificity | Recognizes human alpha Adducin when phosphorylated at Ser481. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
External Links
|
