Applications
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:100-1:200 Western Blot: 1:500-1:1,000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthesized non-phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-SP-F-L)
Form
Supplied as a liquid in PBS, pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of total ADD1 protein.